Method for down-regulating GDF-8 activity
作者： Steen Klysner , Soeren Mouritsen , Torben Halkier
DOI:
关键词: Immunization 、 Nucleic acid 、 Virology 、 Protein tertiary structure 、 Epitope 、 Myostatin 、 Biology 、 Vaccination 、 Immunogen 、 Growth differentiation factor
摘要: Disclosed are novel methods for increasing muscle mass by means of immunization against Growth Differentiation Factor 8 (GDF-8, myostatin). Immunization is preferably effected administration analogues GDF-8 which capable inducing antibody production homologous GDF-8. Especially preferred as an immunogen has been modified introduction one single or a few foreign, immunodominant and promiscuous T-cell epitopes while substantially preserving the tertiary structure Also disclosed nucleic acid vaccination using live vaccines well useful vaccination. Such include identification immunogenic analogues, preparation pharmaceutical formulations, fragments, vectors, transformed cells, polypeptides formulations.
lens.org参考文章(20)
Kirsten Falk, Olaf R�tzschke, Stefan Stevanov�c, G�nther Jung, Hans-Georg Rammensee, Pool sequencing of natural HLA-DR, DQ, and DP ligands reveals detailed peptide motifs, constraints of processing, and general rules Immunogenetics. ,vol. 39, pp. 230- 242 ,(1994) , 10.1007/BF00188785
P M Guyre, D R Gosselin, N Alter, E J Gosselin, K Wardwell, J L Fisher, Enhanced antigen presentation using human Fc gamma receptor (monocyte/macrophage)-specific immunogens. Journal of Immunology. ,vol. 149, pp. 3477- 3481 ,(1992)
S Mouritsen, I Dalum, M R Jensen, H I Elsner, P Hindersson, BREAKING OF B CELL TOLERANCE TOWARD A HIGHLY CONSERVED SELF PROTEIN Journal of Immunology. ,vol. 157, pp. 4796- 4804 ,(1996)
Dominique Poncelet, Luc Grobet, Michel Georges, Mutations in the myostatin gene cause double-muscling in mammals ,(1998)
Alexandra C. McPherron, Se-Jin Lee, Methods of detecting growth differentiation factor-8 Official Gazette of the United States Patent and Trademark Office Patents. ,(2000)
Søren Mouritsen, Henrik Elsner, Inducing antibody response against self-proteins with the aid of foreign t-cell epitopes ,(1994)
Grey Hm, Hoffman S, Southwood S, Kubo Rt, Sette A, Kondo A, Sidney J, Appella E, Chesnut Rw, del Guercio Mf, Several Common HLA-DR Types Share Largely Overlapping Peptide Binding Repertoires Journal of Immunology. ,vol. 160, pp. 3363- 3373 ,(1998)
Pamela A. Wearsch, Laura Voglino, Christopher V. Nicchitta, Structural Transitions Accompanying the Activation of Peptide Binding to the Endoplasmic Reticulum Hsp90 Chaperone GRP94 Biochemistry. ,vol. 37, pp. 5709- 5719 ,(1998) , 10.1021/BI9801006
F. Sinigaglia, M. Guttinger, J. Kilgus, D. M. Doran, H. Matile, H. Etlinger, A. Trzeciak, D. Gillessen, J. R. L. Pink, A malaria T-cell epitope recognized in association with most mouse and human MHC class II molecules. Nature. ,vol. 336, pp. 778- 780 ,(1988) , 10.1038/336778A0
Juergen Hammer, Paola Valsasnini, Khaled Tolba, David Bolin, Jacqueline Higelin, Bela Takacs, Francesco Sinigaglia, Promiscuous and allele-specific anchors in HLA-DR-binding peptides Cell. ,vol. 74, pp. 197- 203 ,(1993) , 10.1016/0092-8674(93)90306-B