A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development
作者： Elizabeth M. Ferguson , Henry J. Leese
DOI: 10.1002/MRD.20494
关键词: Embryo 、 Energy source 、 Oogenesis 、 Biology 、 Mammalian embryology 、 Blastocyst 、 Oocyte 、 Internal medicine 、 Endocrinology 、 Embryo culture 、 Fertilisation 、 Andrology
摘要: The potential role of endogenous triglyceride in bovine oocyte maturation and preimplantation development has been investigated. Bovine immature oocytes were recovered from abattoir-derived ovaries, matured fertilised vitro the zygotes grown to blastocyst stage SOFaaBSA. Methyl palmoxirate (MP) blocks oxidation fatty acids by inhibiting mitochondrial carnitine palmitoyltransferase A. exposed MP during maturation, embryo culture assessed terms oxygen consumption embryos a 4-6 hr incubation period presence as formation cell number. Immature had reduced capacity form blastocysts after fertilisation; same effect was apparent, but lesser extent, development. Oxygen values absence exogenous substrates similar those control medium containing nutrients. MP-inhibited oocytes, mature cleavage stages 64, 45, 12 13%, respectively. data are consistent with for key energy source potentially,
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