Adoptive immunotherapy of human gastric cancer with ex vivo expanded T cells

摘要: Surgical resection of gastric cancer has made significant progress, but majority patients with advanced face relapse and die within five years. In this study, the antitumor activity ex vivo expanded T cells against human was evaluated in vitro vivo. Human peripheral blood mononuclear were cultured IL-2-containing medium anti-CD3 antibody-coated flasks for 5 days, followed by incubation 9 days. The resulting populations mostly CD3+ (97%) comprised 1% CD3−CD56+, 36% CD3+CD56+, 11% CD4+, 80% CD8+. This heterogeneous cell population also called cytokine-induced killer (CIK) cells. CIK strongly produced IFN-γ, moderately TNF-α, not IL-2 IL-4. At an effector-target ratio 30:1, destroyed 58% MKN74 cells, as measured 51Cr-release assay. addition, at doses 3 10 million per mouse inhibited 78% tumor growth nude xenograft assays, respectively. study suggests that may be used adoptive immunotherapy patients.