Differential antigen presentation by heat-treated peripheral blood mononuclear cells and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBV-LCL): Heated EBV-LCL present alloantigen and soluble antigen but are deficient in the stimulation of autologous EBV-LCL primed T cells

摘要: Heat-treated PBM (1 hr at 45 degrees C) cannot present soluble Candida albicans antigens (CAN) or stimulate in the mixed lymphocyte culture (MLC) reaction. This is despite their continued expression of serologically defined class II MHC antigens. In contrast, heat-treated EBV-LCL CAN and allogeneic T cells MLC. Heated strong secondary responses from alloprimed T-cell lines primed test (PLT), while heated only weak responses. To whether this difference was due to a subtle thermal stability functional on cells, were for 1 temperatures C 60 C. Even after treatment C, strongly stimulated alloreactive MLC PLT reactions. are not nonspecifically mitogenic, as they do autologous alloantigens. However, response can be augmented by coculturing with EBV-LCL, suggesting maintain metabolic activity necessary stimulation that deficient PBM. While lines, no longer lines; irradiated both strongly. suggests involvement heat labile antigenic factor recognition but allogenic EBV-LCL.