Applications of whole-cell bacterial sensors in biotechnology and environmental science

摘要: Biosensors have major advantages over chemical or physical analyses with regard to specificity, sensitivity, and portability. Recently, many types of whole-cell bacterial biosensors been developed using recombinant DNA technology. The bacteria are genetically engineered respond the presence chemicals physiological stresses by synthesizing a reporter protein, such as luciferase, β-galactosidase, green fluorescent protein. In addition an overview conventional biosensors, this minireview discusses novel type biosensor photosynthetic bacterium sensor strain crtA gene, which is responsible for carotenoid synthesis, reporter. Since possess wide variety stress-response mechanisms, including antioxidation, heat-shock responses, nutrient-starvation, membrane-damage response elements several proteins can be fused various genes construct versatile set analytes. Portable on-site monitoring freeze-dried biosensing strain, cell array designed high-throughput analysis. Moreover, in future, use single-cell will permit detailed samples. Signals from sensors could detected digital imaging, epifluorescence microscopy, and/or flow cytometry.