Study of breath acetone and its correlations with blood glucose and blood beta-hydroxybutyrate using an animal model with lab-developed type 1 diabetic rats

摘要: Breath acetone has long been a known biomarker for diabetes. However whether breath analysis can be used clinical applications ultimately depends on how concentration is quantitatively and accurately related to an established diagnostic parameter(s). Numerous studies of using human subjects have done, yet this fundamental question remains unaddressed because complex physiological processes various conditions humans are relevant change acetone. We report the first time study its correlations with blood glucose (BG) β-hydroxybutyrate (BHB) animal model rats. 18 non-diabetic healthy rats 20 lab-developed type 1 diabetic (T1D) were as two subject groups. gas samples from collected noninvasive oral intubation method that was compared intrusive time-consuming tracheostomy. concentrations measured cavity ringdown spectroscopy based analyzer without additional sample preparation, pre-concentration, or calibration. The in ranges 1.9–4.3 ppm (part per million by volume) T1D 1.4–2.8 Simultaneous BG BHB levels also obtained. Results show acetone, BG, rat group all significant difference one (P < 0.05). A positive relationship (Pearson’s r = 0.644, P 0.05) between found exist both negative −0.678, only. However, shifts weakly when treated insulin. Furthermore, results multiple linear regression reveal predictive nature rats, which confirms sole subjects. This result suggests large scale help address fundamentals some cases.