GABA synthesis mediated by γ-aminobutanal dehydrogenase in Synechocystis sp. PCC6803 with disrupted glutamate and α-ketoglutarate decarboxylase genes.
作者： Simab Kanwal , Aran Incharoensakdi
DOI: 10.1016/J.PLANTSCI.2019.110287
关键词: Wild type 、 Biochemistry 、 Biology 、 Polyamine 、 Synechocystis 、 Glutamate decarboxylase 、 Citric acid cycle 、 Dehydrogenase 、 Spermidine 、 Glutamate receptor
摘要: A pathway for polyamine-derived GABA synthesis in Synechocystis sp. PCC 6803 was explored by disrupting both the glutamate decarboxylase and α-ketoglutarate genes. The generated Δgad:Δkgd strain had increased intracellular polyamine levels compared to wild type. Gene transcript analysis using RT-PCR indicated that up-regulated expression of a putative gadbh whose gene product, γ-aminobutanal dehydrogenase, would catalyze conversion GABA. with disrupted gabdh showed an increase GABA, glutamate, succinate spermidine levels. These findings provide evidence link between degradation cyanobacteria. This study highlights role dehydrogenase maintaining intact tricarboxylic acid cycle Synechocystis.
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