Electric cell-substrate impedance sensing (ECIS) based real-time measurement of titer dependent cytotoxicity induced by adenoviral vectors in an IPI-2I cell culture model
作者： Jakob Müller , Christian Thirion , Michael W. Pfaffl
DOI: 10.1016/J.BIOS.2010.08.075
关键词: Viral vector 、 RNA interference 、 Cell biology 、 Viral load 、 Electric cell-substrate impedance sensing 、 Biology 、 Multiplicity of infection 、 Transfection 、 Cytotoxicity 、 Cell culture 、 Molecular biology
摘要: Abstract Recombinant viral vectors are widespread tools for transfer of genetic material in various modern biotechnological applications like example RNA interference (RNAi). However, an accurate and reproducible titer assignment represents the basic step most downstream regarding a precise multiplicity infection (MOI) adjustment. As necessary scaffold studies described this work we introduce quantitative real-time PCR (qPCR) based approach particle measurement. Still implicated problem concerning physiological effects is that appliance often attended by toxic on individual target. To determine critical dose leading to cell death developed electric cell-substrate impedance sensing (ECIS) assay. With ECIS technology change current flow through culture medium array plate measured non-invasive manner, visualizing attachment, cell–cell contacts or proliferation. Here describe potential online measurement technique vitro model using porcine ileal epithelial line IPI-2I combination with adenoviral transfection vector (Ad5-derivate). This shows clear dose-depending effect, as amount applied virus highly correlates ( p
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