Development of a versatile reporter assay for studies of retinol uptake and metabolism in vivo.

摘要: The two isomers of retinoic acid (RA), all-trans RA and 9-cis RA, are produced in several tissues order to allow specific control target gene transcription. Given the high potency these receptor ligands, it seems likely that cellular uptake metabolic activation precursor, retinol (vitamin A), should be a highly regulated process. Several dehydrogenases components involved downstream events have been identified partially characterized. However, less is known about retinol, isomerase activity giving rise 11-cis branches pathway. In this work, we show biosynthesis pathway can fully reconstituted cultured HEK293A cells expressing reporter system, including an endogenous converting into retinol. This assay allows for functional studies components, as well screening yet unidentified genes addition free find take up from plasma binding protein (RBP) by mechanism efficiently inhibited blocking antibodies, suggesting may involve receptor. We also demonstrate overexpression CRBPI drive accumulation intracellular unbound added medium. Thus, versatile used study aspects metabolism vivo.