Idiotypic DNA vaccines against B-cell lymphoma

摘要: Idiotypic antigens are clearly defined tumor-associated protein antigens, which can induce protective immunity against lymphoma. Because each patient requires an individual vaccine, idiotypic also provide ideal candidates for exploring the feasibility of replacing by DNA vaccines. Component variable region genes be identified in patients' tumor biopsies and rapidly assembled as scFv sequences. These used to produce recombinant bacteria, or direct naked A preliminary small trial vaccines chemotherapy-resistant patients with lymphoma has begun. Intramuscular vaccination a mouse model induces low levels anti-idiotypic antibody serum. Levels increased dramatically co-injection plasmids encoding either IL-2 GM-CSF, specific proliferative T cells induced. However remains demonstrated, possible reason this may lie continued secretion antigen blocks activity formation immune complexes. Methods regulating required before category fully exploited vaccine. The power technology should allow analysis manipulation pathways presentation maximal therapeutic attack on neoplastic B cells. In addition, presents application wide range human tumors known harbor potential antigens.