Detection of petunia vein-clearing virus: model for the detection of DNA viruses in plants with homologous endogenous pararetrovirus sequences.

摘要: A number of cases plant virus sequence integration into host genome have been reported. In at least two cases, endogenous pararetrovirus sequences are correlated strongly with subsequent episomal infection and there is circumstantial evidence that this also occurs for Petunia vein-clearing (PVCV). The detection viruses a critical component health therefore, it important to diagnostic procedures differentiate between the encapsidated viral DNA homologous in genome. PCR-based methods targeted PVCV tested particular attention was paid design controls would indicate existence reaction. use ion-exchange chromatography partial purification from other cellular components, including chromosomal DNA, described. used illustrate general principles specific infections plants carry their genomes.