TNF-related apoptosis-inducing ligand promotes human preadipocyte proliferation via ERK1/2 activation
作者： Jan‐Bernd Funcke , Verena Zoller , Muad Abd El Hay , Klaus‐Michael Debatin , Martin Wabitsch
DOI: 10.1096/FJ.14-267278
关键词: Apoptosis 、 Endocrinology 、 Small hairpin RNA 、 Inflammation 、 Internal medicine 、 Biology 、 Fibrosis 、 Cell growth 、 Cellular homeostasis 、 Adipose tissue 、 Cell biology 、 Caspase 8
摘要: Upon obesity, adipose tissue is excessively expanded and characterized by pathologic processes like hypoxia, fibrosis, inflammation. Death ligands belonging to the TNF superfamily such as TNF-α are important contributors these derangements exert a pronounced influence on metabolic cellular homeostasis of tissue. Here, we sought identify effect death ligand TNF-related apoptosis-inducing (TRAIL) precursor cell pool therefore investigated its preadipocyte proliferation. Treatment human preadipocytes with TRAIL resulted in time- dose-dependent increase proliferation (EC50 3.4 ng/ml) comparable IGF-1. Although no apoptosis was observed, triggered rapid cleavage caspase-8 -3. Neither inhibition caspase activity zVAD.fmk (20 µM) nor ablation expression lentivirus-delivered small hairpin RNA (shRNA) abolished proliferative response. delayed sustained...
fasebj.org参考文章(55)
Larisa L. Belyanskaya, Algirdas Ziogas, Sally Hopkins-Donaldson, Stefanie Kurtz, Hans-Uwe Simon, Rolf Stahel, Uwe Zangemeister-Wittke, TRAIL-induced survival and proliferation of SCLC cells is mediated by ERK and dependent on TRAIL-R2/DR5 expression in the absence of caspase-8. Lung Cancer. ,vol. 60, pp. 355- 365 ,(2008) , 10.1016/J.LUNGCAN.2007.11.005
J Lemke, S von Karstedt, J Zinngrebe, H Walczak, Getting TRAIL back on track for cancer therapy. Cell Death & Differentiation. ,vol. 21, pp. 1350- 1364 ,(2014) , 10.1038/CDD.2014.81
Soo Hwan Pai, Jong Weon Choi, Jung Soo Song, Associations of Serum TRAIL Concentrations, Anthropometric Variables, and Serum Lipid Parameters in Healthy Adults Annals of Clinical and Laboratory Science. ,vol. 34, pp. 400- 404 ,(2004)
Fernando O. Martinez, Siamon Gordon, Massimo Locati, Alberto Mantovani, Transcriptional Profiling of the Human Monocyte-to-Macrophage Differentiation and Polarization: New Molecules and Patterns of Gene Expression Journal of Immunology. ,vol. 177, pp. 7303- 7311 ,(2006) , 10.4049/JIMMUNOL.177.10.7303
D. Samocha-Bonet, V. D. Dixit, C. R. Kahn, R. L. Leibel, X. Lin, M. Nieuwdorp, K. H. Pietiläinen, R. Rabasa-Lhoret, M. Roden, P. E. Scherer, S. Klein, E. Ravussin, Metabolically healthy and unhealthy obese - the 2013 Stock Conference report Obesity Reviews. ,vol. 15, pp. 697- 708 ,(2014) , 10.1111/OBR.12199
Hans Hauner, Thomas Skurk, Martin Wabitsch, Cultures of Human Adipose Precursor Cells Adipose Tissue Protocols. ,vol. 155, pp. 239- 247 ,(2001) , 10.1385/1-59259-231-7:239
Jacques Morel, Rachel Audo, Michael Hahne, Bernard Combe, Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) Induces Rheumatoid Arthritis Synovial Fibroblast Proliferation through Mitogen-activated Protein Kinases and Phosphatidylinositol 3-Kinase/Akt Journal of Biological Chemistry. ,vol. 280, pp. 15709- 15718 ,(2005) , 10.1074/JBC.M414469200
D. B. Hausman, M. DiGirolamo, T. J. Bartness, G. J. Hausman, R. J. Martin, The biology of white adipocyte proliferation. Obesity Reviews. ,vol. 2, pp. 239- 254 ,(2001) , 10.1046/J.1467-789X.2001.00042.X
Paola Secchiero, Giorgio Zauli, The Puzzling Role of TRAIL in Endothelial Cell Biology Arteriosclerosis, Thrombosis, and Vascular Biology. ,vol. 28, ,(2007) , 10.1161/ATVBAHA.107.158451
Hubert Kasperczyk, Katia La Ferla-Brühl, Mike Andrew Westhoff, Lars Behrend, Ralf Michael Zwacka, Klaus-Michael Debatin, Simone Fulda, Betulinic acid as new activator of NF-kappaB: molecular mechanisms and implications for cancer therapy. Oncogene. ,vol. 24, pp. 6945- 6956 ,(2005) , 10.1038/SJ.ONC.1208842