High-performance liquid chromatography-mass spectrometry of an osteocalcin derivative.
作者： Hanna Vähätalo , Seppo Auriola , Seppo Lapinjoki
DOI: 10.1016/S0021-9673(98)01040-1
关键词: High-performance liquid chromatography 、 Electrospray ionization 、 Electrospray 、 Chemistry 、 Peptide 、 Derivative (chemistry) 、 Chromatography 、 Osteocalcin 、 Chemical stability 、 Mass spectrometry
摘要: High-performance liquid chromatography (HPLC) was combined on-line with electrospray ionization mass spectrometry (ESI-MS) for structural analysis of a synthetic osteocalcin derivative and its degradation products. Initial determination amino acid sequence the peptide performed after tryptic degradation. Hydrolytic studied under different pH conditions: 2, 7 10 at 60°C up to 20 h. According HPLC-ESI-MS results, chemical stability dependent on pH. Two major products number other fragments were obtained in acidic solution, whereas molecule rather stable neutral alkaline conditions.
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