High-performance liquid chromatography--mass spectrometry and electron-capture dissociation tandem mass spectrometry of osteocalcin. Determination of gamma-carboxyglutamic acid residues.
作者： Hanna Niiranen , Bogdan A Budnik , Roman A Zubarev , Seppo Auriola , Seppo Lapinjoki
DOI: 10.1016/S0021-9673(02)00451-X
关键词:
摘要: Two mass spectrometry methods, high-performance liquid chromatography combined on-line with electrospray ionization (HPLC-ESI-MS) and electron-capture (EC) dissociation tandem (MS-MS), were applied for structural analysis of bovine human osteocalcins. Osteocalcin contains gamma-carboxyglutamic acid (Gla) residues, which bind metal ions, among its amino acids. Ethylenediaminetetraacetic (EDTA) was added to all samples in order chelate bound ions. After elimination interfering ions MS spectra became uncomplicated interpret. EDTA is incompatible ESI it removed from using either HPLC or micropurification method. The number Gla residues varies osteocalcin. These subforms, contain different amounts separated the HPLC-ESI-MS In determine locations osteocalcin, contained two MS-MS method successfully applied.
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