Acanthamoeba spp. as vehicle and reservoir of adenoviruses

摘要: Adenoviruses are important pathogens which responsible for human enteritic, respiratory and eye infections. These viruses have been found to be prevalent in several natural artificial water reservoirs worldwide. Free-living amoebae (FLA) recovered from similar reservoirs, it has shown that FLA may act as or vehicles of various microorganisms living the same environment. To examine ability harbour adenoviruses, an vitro study was conducted. Several Acanthamoeba strains were ‘co-cultivated’ with adenoviruses (adenoviruses 11 41), grown on A549 cells, using a proven test protocol. After phagocytosis ingestion, could within cytoplasm trophozoites. The intake into trophozoites demonstrated castellanii strain help fluorescence microscopy electron microscopy. An adenovirus DFA kit, utilizes direct immunofluorescent antibody technique identifying infected tissue cultures, used. In our study, incorporated host (Acanthamoeba sp. Grp. II, three strains). So far, there only few publications concerning relationship free-living viruses; one these described detection acanthamoebae molecular biological methods. We conducted this descriptive further association between viable FLA. knowledge, is first demonstrate directly vectors vehicles. Therefore, we concluded appear able carriers thus play certain role dispersal adenoviruses.