Mitogenic effects of Brazilian arthropod venom on isolated islet beta cells: in vitro morphologic ultrastructural and functional studies.

摘要: Background One of the major pitfalls associated with use isolated adult islets Langerhans9 cells is their minimal mitotic capacity. Consequently, maintenance a steady viable islet cell mass very difficult. To explore how to enhance beta-cell mitoge-nesis, we have examined effects venom fractions extracted from Brazilian scorpion on morphologic and functional patterns. The was previously known induce nesidioblas-tosis-like chronic hypoglycemia pancreatitis in animal models. Methods Venom purified Tityus bahiensis were incubated batches rat islets, while examination, glucose-stimulated insulin release, content, messenger ribonucleic acid (mRNA) carried out early during incubation. On fixation double fluorescence immunolabeling (rhodamine for anti-insulin monoclonal antibodies; fluorescein anti-5-bromodeoxyuridine), preparations imaged by confocal laser microscopy (CLM) morphome- trie quantification mitoses. Insulin recovery mRNA also assessed at 21 days culture. Results Under CLM rate significantly rose 1 12.8% venom-exposed islets. At day 7, release content lower than control However, culture, response static incubation glucose higher controls (p Conclusions Incubation induced rapid significant increase proliferation not short-term secretion. latter fully resumed overcame later possibly after completion expansion process.