Sensitive Photonic System to Measure Oxidative Potential of Airborne Nanoparticles and ROS Levels in Exhaled Air

摘要: Abstract A photonic system has been developed that enables sensitive quantitative determination of reactive oxygen species (ROS) – mainly hydrogen peroxide (H 2 O ) in aerosol samples such as airborne nanoparticles and exhaled air from patients. The detection principle relies on the amplification absorbance under multiple scattering conditions due to optical path lengthening [1,2] . In this study, presence cellulose membrane acts random medium into glass cell considerably improved sensitivity based colorimetric FOX assay (FeII/orange xylenol). Despite loss volume (cellulose occupies 75% volume) limit is enhanced by one order magnitude reaching value 9 nM equivalents). Spectral analysis performed automatically with a periodicity 5 15 s, giving rise real-time ROS measurements. Moreover, elution sample collection chamber via micro-diffuser (impinger) contained or generated samples. As proof-of-concept was used both traffic pollution breath lung inflammation biomarkers.