Simultaneous production of acetate and methane from glycerol by selective enrichment of hydrogenotrophic methanogens in extreme-thermophilic (70 °C) mixed culture fermentation
作者： Fang Zhang , Yan Zhang , Yun Chen , Kun Dai , Mark CM van Loosdrecht
DOI: 10.1016/J.APENERGY.2015.03.104
关键词: Thermophile 、 Biogas 、 Thermoanaerobacter 、 Fermentation 、 Biochemistry 、 Biology 、 Glycerol 、 Archaea 、 Food science 、 Biodiesel production 、 Methane
摘要: Abstract The feasibility of simultaneous production acetate and methane from glycerol was investigated by selective enrichment hydrogenotrophic methanogens in an extreme-thermophilic (70 °C) fermentation. Fed-batch experiments showed produced at the concentration up to 13.0 g/L. A stable operation continuous stirred tank reactor (CSTR) reached within 100 days. Acetate accounted for more than 90 w/w% metabolites fermentation liquid. yields were close theoretical with 0.74–0.80 mol-methane/mol-glycerol 0.63–0.70 mol-acetate/mol-glycerol. obtained microbial community characterized. Hydrogenotrophic methanogens, mainly Methanothermobacter thermautotrophicus formed 93% methanogenogenic community. This confirms that a high temperature could effectively select methanogenic archaea. Thermoanaerobacter spp. main bacterium forming 91.5% bacterial population. work demonstrated conversion byproduct biodiesel production, glycerol, as chemical biogas energy generation.
elsevier.com
sci-hub.se 参考文章(40)
Takuro Nunoura, Hanako Oida, Junichi Miyazaki, Ai Miyashita, Hiroyuki Imachi, Ken Takai, Quantification of mcrA by fluorescent PCR in methanogenic and methanotrophic microbial communities. FEMS Microbiology Ecology. ,vol. 64, pp. 240- 247 ,(2008) , 10.1111/J.1574-6941.2008.00451.X
Andrew Eaton, A.E. Greenberg, Lenore S. Clesceri, Standard methods for the examination of water and wastewater Published in <b>1998</b> in Washington DC) by American public health association. ,(1992)
Anoop Singh, Stig Irving Olsen, A critical review of biochemical conversion, sustainability and life cycle assessment of algal biofuels Applied Energy. ,vol. 88, pp. 3548- 3555 ,(2011) , 10.1016/J.APENERGY.2010.12.012
Lise Larsen, Peter Nielsen, B. K. Ahring, Thermoanaerobacter mathranii sp. nov., an ethanol-producing, extremely thermophilic anaerobic bacterium from a hot spring in Iceland. Archives of Microbiology. ,vol. 168, pp. 114- 119 ,(1997) , 10.1007/S002030050476
J. Yan, T. Lin, Biofuels in Asia Applied Energy. ,vol. 86, ,(2009) , 10.1016/J.APENERGY.2009.07.004
Helena Moralejo-Gárate, Robbert Kleerebezem, Anuska Mosquera-Corral, Mark C.M. van Loosdrecht, Impact of oxygen limitation on glycerol-based biopolymer production by bacterial enrichments. Water Research. ,vol. 47, pp. 1209- 1217 ,(2013) , 10.1016/J.WATRES.2012.11.039
Gopi Krishna Kafle, Sang Hun Kim, None, Anaerobic treatment of apple waste with swine manure for biogas production: Batch and continuous operation Applied Energy. ,vol. 103, pp. 61- 72 ,(2013) , 10.1016/J.APENERGY.2012.10.018
Rudolf K. Thauer, Anne-Kristin Kaster, Henning Seedorf, Wolfgang Buckel, Reiner Hedderich, Methanogenic archaea: ecologically relevant differences in energy conservation Nature Reviews Microbiology. ,vol. 6, pp. 579- 591 ,(2008) , 10.1038/NRMICRO1931
SpyrosG. Pavlostathis, TerryL. Miller, MeyerJ. Wolin, Cellulose fermentation by continuous cultures of Ruminococcus albus and Methanobrevibacter smithii Applied Microbiology and Biotechnology. ,vol. 33, pp. 109- 116 ,(1990) , 10.1007/BF00170581
Niclas Krakat, Antje Westphal, Kaspar Satke, Stefan Schmidt, Paul Scherer, The microcosm of a biogas fermenter: Comparison of moderate hyperthermophilic (60°C) with thermophilic (55°C) conditions Engineering in Life Sciences. ,vol. 10, pp. 520- 527 ,(2010) , 10.1002/ELSC.201000064