Anti-H5N1 virus metabolites from the Red Sea soft coral, Sinularia candidula

摘要: Abstract A novel polyhydroxylated sterol, 3β-25-dihydroxy-4-methyl-5α,8α-epidioxy-2-ketoergost-9-ene ( 1 ) along with three new ceramides, N -[(2 S ,3 R , E )-1,3-dihydroxyhexacos-4-en-2-yl]icosanamide 2 ), ,4 )-1,3,4-trihydroxyhexacosan-2-yl]icosanamide 3 and )-2′-hydroxy- )-1,3,4-trihydroxypentacosan-2-yl] nonadecanamide 4 were isolated from the Red Sea soft coral, Sinularia candidula. Complete structure elucidation was achieved through extensive spectroscopic analysis including one- two-dimensional nuclear magnetic resonance spectroscopy. candidula extracts of different polarities as well pure compounds screened against highly pathogenic H5N1 avian influenza strain using plaque inhibition assay in MDCK. The results indicated that coral possessed potent antiviral activity (100% at a concentration 1 μg/mL). isolates showed reduction virus titer by 55.16%, 48.81%, 10.43%, 15.76% 1 ng/mL, respectively.