Reversed-phase chromatography of some aromatic hydrocarbons

摘要: Abstract The chromatographic behaviour of some aromatic hydrocarbons has been studied on columns Zipax Permaphase ODS in water—methanol mixed solvents. increase retention with the number rings a molecule is noted. A simple relation between k ′ (measure retention) and empirical formulae for unsubstituted polynuclear suggested. substitution alkyl side chains to an nucleus causes further retention. Comparisons show that aliphatic carbon atoms are more effective at increasing than their counterparts. Chromatographic alkylbenzenes can be related chain. These relationships together linking mobile phase composition might used give approximate idea value hydrocarbon given its structure. content could estimated from positions these reversed-phase chromatograms. Furthermore, identity aralkyl hydrocarbon, extent also estimated. Such structural assignments reinforced by spectroscopic examination separated fractions.