Morphine promotes the growth of HIV-1 in human peripheral blood mononuclear cell cocultures.

摘要: Because morphine has been shown to alter the function of human T lymphocytes and monocytes, we postulated that would promote growth HIV-1 in these cells. To test this hypothesis, a coculture assay was used consisting phytohemagglutinin (PHA)-activated peripheral blood mononuclear cells (PBMC) from normal donors PBMC which had infected with viral isolate an asymptomatic patient, HIV-1AT. The HIV-1AT, as reflected by concentration p24 antigen supernatants, markedly increased cocultures contained morphine. A bell-shaped dose-response curve observed three- fourfold at 10(-12) M. Augmentation HIV-1AT required interaction PHA-activated donor PBMC. Furthermore, potentiation stereospecific antagonized naloxone beta-funaltrexamine indicating involvement opiate receptor mechanism. These findings provide additional explanation how opiates could act cofactor pathogenesis intravenous drug users.