Predictive model of conjugative plasmid transfer in the rhizosphere and phyllosphere.

摘要: A computer simulation model was used to predict the dynamics of survival and conjugation Pseudomonas cepacia (carrying transmissible recombinant plasmid R388:Tn1721) with a nonrecombinant recipient strain in simple rhizosphere phyllosphere microcosms. Plasmid transfer rates were derived for mass action model, donor modeled as exponential growth decay processes or both. Rate parameters from laboratory studies which strains incubated test tubes peat-vermiculite solution on excised radish bean leaves petri dishes. The predicted donor, recipient, transconjugant populations hourly time steps. It tested microcosm planted seeds inoculated leaf surfaces plants also growing Bacteria periodically enumerated selective media over 7 14 days. When 10(6) 10(8) CFU/g (wet weight) plant soil, about 10(1) 10(4) observed after 1 day. An initial rapid increase subsequent decline numbers transconjugants correctly predicted.