The miR‐143/145 Cluster, a Novel Diagnostic Biomarker in Chondrosarcoma, Acts as a Tumor Suppressor and Directly Inhibits Fascin‐1

摘要: Chondrosarcoma is the second most frequent bone sarcoma. Due to inherent chemotherapy and radiotherapy resistance absence of known therapeutic targets, clinical management limited surgical resection. Consequently, patients with advanced disease face a poor prognosis. Hence, elucidating regulatory networks governing chondrosarcoma pathogenesis vital for development effective strategies. Here, miRNA mRNA next generation sequencing different subtypes human chondrogenic tumors in combination silico bioinformatics tools were performed aim identify key molecular factors. We identified miR-143/145 cluster levels inversely correlate tumor grade. This deregulation was echoed plasma we provided first evidence that circulating miR-145 potential noninvasive diagnostic biomarker can be valuable as an indicator improve currently challenging diagnosis cartilaginous tumors. Additionally, artificial upregulation both miRNAs impelled potent suppressor effect vitro vivo orthotopic xenograft mouse model. A combined silico/sequencing approach revealed FSCN1 direct target miR-143/145, its depletion phenotypically resembled vitro. Last, malignancy-promoting factor associated aggressive progression. Our findings underscore miR-143/145/FSCN1 important players may potentially open new avenues specific intervention options. © 2020 American Society Bone Mineral Research.