Interface Fluid Syndrome in Human Eye Bank Corneas after LASIK: Causes and Pathogenesis

摘要: Purpose To evaluate the effects of corneal edema on human donor corneas that had previous LASIK using a laboratory model with histologic and ultrastructural correlations. Design Experimental study. Participants Thirty eye bank from 15 donors (mean age ± standard deviation, 49.9±8.9 years) who surgery (2–8 years before death). Methods The were mounted in an artificial anterior chamber endothelium was perfused for up to 5.0 hours 0.9% saline solution (endothelial cell damage group) or BSS Plus at pressure mmHg (control group), 55 (high-pressure group). evaluated by confocal specular microscopy before, during, end experimental period. Subsequently, specimens light electron microscopy. Main Outcome Measures Corneal thickness, reflectivity, histology, ultrastructure. Results Endothelial resulted increased (141.5±38.8 μm) total thickness relative controls (52.3±33.7 μm), whereas high decreased (24.8±14.1 controls. This ultimately due swelling interface both groups residual stromal bed (RSB) endothelial group compression RSB and, possibly, flap high-pressure group. A significant increase reflectivity occurred groups, primarily varying degrees fluid accumulation associated hydropic keratocyte degeneration, as well only Conclusions After surgery, edematous preferentially hydrate swell paracentral central wound, commonly resulting hazy appearance degeneration. More severe is characterized formation optically empty space corresponding pocket. spectrum syndrome can be described 3 stages.