Neurogenesis in the Dentate Gyrus of the Adult Tree Shrew Is Regulated by Psychosocial Stress and NMDA Receptor Activation

摘要: These studies were designed to determine whether adult neurogenesis occurs in the dentate gyrus of tree shrew, an animal phylogenetically between insectivores and primates, explore possibility that this process is regulated by stressful experiences NMDA receptor activation. We performed immunohistochemistry for cell-specific markers thymidine analog bromodeoxyuridine (BrdU), a marker DNA synthesis labels proliferating cells their progeny, on brains shrews subjected psychosocial stress or antagonist treatment. Cells incorporated BrdU primarily located subgranular zone, had morphological characteristics granule neuron precursors, appeared divide within 24 hr after injection. Three weeks injection, BrdU-labeled neuronal morphology, expressed specific enolase, into cell layer. Vimentin-immunoreactive radial glia observed with bodies zone processes extending Exposure acute resulted rapid decrease number gyrus. In contrast, blockade receptors, MK-801, increase results indicate shrew experience Furthermore, we suggest these may be common most mammalian species.