Whole Body Composition of Rats Determined by Dual Energy X-Ray Absorptiometry Is Correlated with Chemical Analysis

摘要: The use of dual energy X-ray absorptiometry (DXA) is increasing in animal research, but data comparing whole body composition by DXA and chemical analysis (CHEM) rats are limited. Lean obese Zucker female were used to compare (QDR1000W, Hologic, Waltham, MA) values with CHEM for percent fat (%FATDXA, %FATCHEM), lean mass (%PROTDXA, %PROTCHEM) bone mineral content (%BMCDXA, %ASHCHEM). Four groups (n = 9) tested differences due consumption a 100 g guar gum supplement/kg see if was as sensitive detecting induced diet. study analyzed using split-plot ANOVA where the main plot 2 x factorial phenotype (obese or lean) treatment (guar control) effects, subplot method (DXA CHEM), which treated repeated measure. Absolute differed significantly (P < 0.0001) between two methods %FATDXA consistently higher than %FATCHEM. There not statistically significant difference %PROT 0.13). Values %BMCDXA 0.0049) lower %ASHCHEM values. diet detected similarly CHEM. Significant correlations found %FAT (r 0.99), 0.96) %BMC ASH 0.81). Bland-Altman plots showed good agreement methods, regression equations developed estimate from readings. may provide an alternative assessing changes composition.