The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway.
作者： Cecilia Bucci , Robert G. Parton , Ian H. Mather , Henk Stunnenberg , Kai Simons
DOI: 10.1016/0092-8674(92)90306-W
关键词: Cell biology 、 Endocytosis 、 Biology 、 Receptor-mediated endocytosis 、 Endocytic cycle 、 Vesicular transport protein 、 RAB7A 、 GTP-binding protein regulators 、 Endosome 、 EEA1
摘要: We have investigated the in vivo functional role of rab5, a small GTPase associated with plasma membrane and early endosomes. Wild-type rab5 or rab5-ile133, mutant protein defective GTP binding, was overexpressed baby hamster kidney cells. In cells expressing rab5ile 133 protein, rate endocytosis decreased by 50% compared normal, while recycling not significantly affected. The morphology endosomes also drastically changed which induced accumulation tubules vesicles at periphery cell. Surprisingly, overexpression wild-type accelerated uptake endocytic markers led to appearance atypically large conclude that is rate-limiting component machinery regulating kinetics traffic pathway.
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M. Zerial, P. Melancon, C. Schneider, H. Garoff, The transmembrane segment of the human transferrin receptor functions as a signal peptide. The EMBO Journal. ,vol. 5, pp. 1543- 1550 ,(1986) , 10.1002/J.1460-2075.1986.TB04395.X
B. Podbilewicz, I. Mellman, ATP and cytosol requirements for transferrin recycling in intact and disrupted MDCK cells. The EMBO Journal. ,vol. 9, pp. 3477- 3487 ,(1990) , 10.1002/J.1460-2075.1990.TB07556.X
J.E. Gruenberg, K.E. Howell, Reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. The EMBO Journal. ,vol. 5, pp. 3091- 3101 ,(1986) , 10.1002/J.1460-2075.1986.TB04615.X
T Kobayashi, R E Pagano, Lipid transport during mitosis. Alternative pathways for delivery of newly synthesized lipids to the cell surface. Journal of Biological Chemistry. ,vol. 264, pp. 5966- 5973 ,(1989) , 10.1016/S0021-9258(18)83644-4
N. C. Walworth, B. Goud, A. K. Kabcenell, P. J. Novick, Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic. The EMBO Journal. ,vol. 8, pp. 1685- 1693 ,(1989) , 10.1002/J.1460-2075.1989.TB03560.X
A Mizoguchi, S Kim, T Ueda, A Kikuchi, H Yorifuji, N Hirokawa, Y Takai, Localization and subcellular distribution of smg p25A, a ras p21-like GTP-binding protein, in rat brain. Journal of Biological Chemistry. ,vol. 265, pp. 11872- 11879 ,(1990) , 10.1016/S0021-9258(19)38480-7
G G Sahagian, C J Steer, Transmembrane orientation of the mannose 6-phosphate receptor in isolated clathrin-coated vesicles. Journal of Biological Chemistry. ,vol. 260, pp. 9838- 9842 ,(1985) , 10.1016/S0021-9258(17)39312-2
A Ciechanover, A L Schwartz, A Dautry-Varsat, H F Lodish, Kinetics of internalization and recycling of transferrin and the transferrin receptor in a human hepatoma cell line. Effect of lysosomotropic agents. Journal of Biological Chemistry. ,vol. 258, pp. 9681- 9689 ,(1983) , 10.1016/S0021-9258(17)44551-0
J F Rodriguez, R Janeczko, M Esteban, Isolation and characterization of neutralizing monoclonal antibodies to vaccinia virus. Journal of Virology. ,vol. 56, pp. 482- 488 ,(1985) , 10.1128/JVI.56.2.482-488.1985
F G Falkner, B Moss, Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. Journal of Virology. ,vol. 62, pp. 1849- 1854 ,(1988) , 10.1128/JVI.62.6.1849-1854.1988