(18)F-misonidazole PET imaging of hypoxia in micrometastases and macroscopic xenografts of human non-small cell lung cancer: a correlation with autoradiography and histological findings.

摘要: The objective of this study was to determine whether (18)F-misonidazole could detect hypoxia in macroscopic and microscopic tumors mice. In nude mice, subcutaneous xenografts peritoneal metastases were generated utilizing human non-small cell lung cancer A549 HTB177 cells. Animals co-injected with (18)F-misonidazole, pimonidazole bromodeoxyuridine, tumor perfusion assessed by Hoechst 33342 injection. intratumoral distribution determined micro-PET scan autoradiography. Pimonidazole, bromodeoxyuridine detected immunohistochemistry on the autoradiography sections. Submillimeter micrometastases found be severely hypoxic. both models, PET images displayed significant uptake, its non-uniform these tumors. frozen sections, digital revealed similar distributions glucose transporter-1, Bromodeoxyuridine stained-positive proliferative regions well perfused, as judged 33342, low accumulation. uptake stroma necrotic zones well. Microscopic are is capable image noninvasively not only but also growing Accordingly, may a promising agent burden micrometastatic diseases.