Cytokeratin-20 and Survivin-Expressing Circulating Tumor Cells Predict Survival in Metastatic Colorectal Cancer Patients by a Combined Immunomagnetic qRT-PCR Approach

摘要: Circulating tumor cells (CTC) express epithelial and stem cell–like genes, though current approved detection methods mainly use markers. We optimized a CTC isolation method that could capture their molecular heterogeneity predict overall survival (OS) in metastatic colorectal cancer (mCRC) patients receiving various chemotherapy regimens. combined immunomagnetic enrichment of CD45-negative, EpCAM-positive circulating with qRT-PCR amplification CK20 survivin expression 88 mCRC 20 healthy controls. then evaluated the prognostic value baseline patients. The presence elevated or distinguished from controls sufficient sensitivity (79.6%) specificity (85%). In univariate analysis, high CTC- (9 vs. 33.2+ months, log-rank P < 0.001) (10 = 0.032) had significantly worse median OS than those low either marker. multivariable group shortened (HR, 3.11; adjusted 0.01), there was trend toward inferior 1.76; 0.099). Patients compared both markers 4.39; 95% confidence interval, 1.56–12.35; 0.005). Colorectal CTCs can be reliably isolated using cell may effectively chemotherapy. Mol Cancer Ther; 14(10); 1–8. ©2015 AACR.