Challenges in the enumeration and phenotyping of CTC.

摘要: Abstract PURPOSE: Presence of circulating tumor cells (CTC) in metastatic carcinoma is associated with poor survival. Phenotyping and genotyping CTC may permit "real-time" treatment decisions, provided CTCs are available for examination. Here, we investigate what needed to detect all patients. EXPERIMENTAL DESIGN: enumerated 7.5 mL blood together survival from 836 patients breast, colorectal, prostate cancer were used predict the concentration 42% these whom no found establish relation Influence different definitions investigated by automated cell recognition a flow cytometric assay without an enrichment or permeabilization step. RESULTS: A log-logistic regression log yielded good fit frequency distribution. Extrapolation volume 5 L predicted that 99% had at least one before therapy initiation. Survival EpCAM+, cytokeratin+, CD45- nucleated reduced 6.6 months each 10-fold increase. Using cytometry, potential three-fold recovery improvement not sufficient blood. CONCLUSIONS: present prostate, colorectal their proportional To serve as liquid biopsy majority patients, substantial yield needed, which can only be achieved dramatic increase sample volume. Clin Cancer Res; 18(20); 5711-8. ©2012 AACR. PMID:23014524[PubMed - process]