作者: P Schofield , A N Pell , D O Krause
DOI: 10.1128/AEM.63.3.1143-1147.1997
关键词: Bacteria 、 Rumen 、 Microbial ecology 、 16S ribosomal RNA 、 Biology 、 Molecular beacon 、 Solution hybridization 、 Molecular probe 、 Fibrobacter succinogenes 、 Biochemistry
摘要: Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested. Membrane hybridizations gave similar results in competition experiments with cocultures R. 8 F. succinogenes S85.