Decreased plasma folate concentration in young and elderly healthy subjects after a short-term supplementation with isotretinoin.

摘要: Background  In the last two decades, there has been an increasing use of isotretinoin (13-cis-retinoic acid or 13-CRA) for treatment severe, and recently mild moderate, acne in Westernized populations. Recent human animal studies emphasized alterations caused by 13-CRA administration on folate-dependent, one-carbon metabolism. Folate deficiency subsequent hyperhomocysteinemia increase risk degenerative diseases. Objectives  We determine whether a short-term supplementation with alters folate status homocysteinemia young elderly healthy subjects. Methods  Twenty 20 (age mean, 26.1 65.4 years, respectively) male volunteers were supplemented ~0.5 mg/kg/day 28 days. Fasting plasma concentrations 13-CRA, 5-methyltetrahydrofolate (5-mTHF) as main circulating form folate, homocysteine (Hcy), well haematologic parameters biochemical markers liver renal function, measured at baseline end supplementation. Statistical analyses carried out using two-way anova standard tests. Results  both groups, dramatic concentration its derivatives. It also led to significant increases serum triglyceride (P < 0.0001) creatinine (P = 0.002) γ-glutamyltranspeptidase activity (P = 0.0001) decrease level urea (P = 0.027). However, latter four remained within normal ranges. These changes accompanied 17.7% 13.5% 5-mTHF (P = 0.001) volunteers, respectively. Supplementation did not cause variations their Hcy concentration. parameter seemed respond differently each group age (P = 0.046). Conclusions  Our data indicate that 28-day old individuals. This stresses necessity studying long-term effects retinoid therapy patients, given alteration is known diseases.