Expression and active testing of VP7 from GCRV (Grass carp reovirus) fused with cholera toxin B subunit in rice calli.

摘要: Abstract Grass carp reovirus (GCRV) is one of the most serious pathogens threatening grass (Ctenopharyngodon idellus) production and results in high mortality China. VP7 from GCRV involved viral infection could be suitable for developing vaccines control infection. To obtain a genetically engineered vaccine plant-based oral to evaluate their immune efficacy as an against GCRV, cholera toxin B subunit (CTB) Vibrio cholerae fused (CTB-VP7) was transformed into BL21(DE3) expression. SDS-PAGE Western blotting showed that purified CTB-VP7 fusion protein (rCTB-VP7) approximately 49.0 kDa. Meanwhile, rice callus cells by Agrobacterium tumefaciens-mediated gene transformation. integrated nuclear genome PCR, mRNA transcripts were detected. ELISA blot analyses revealed expressed lines. The level expression determined 1.54% ± 0.43 total soluble protein. binding affinity monosialoganglioside(GM1), receptor CTB. higher towards GM1 compared rCTB-VP7. bonded with different affinities under temperatures. Maximum reported occur within 2 h at 37 °C, half remained at 25 °C. Our suggest produced calli, increasing possibility edible plants can employed mucosal protection aquaculture.