Translation of Small Open Reading Frames within Unannotated RNA Transcripts in Saccharomyces cerevisiae
作者： Jenna E. Smith , Juan R. Alvarez-Dominguez , Nicholas Kline , Nathan J. Huynh , Sarah Geisler
DOI: 10.1016/J.CELREP.2014.05.023
关键词: Ribosome profiling 、 Biology 、 RNA 、 Gene expression 、 Open reading frame 、 Ribosome 、 Genetics 、 Translation (biology) 、 Protein biosynthesis 、 Polysome
摘要: Summary High-throughput gene expression analysis has revealed a plethora of previously undetected transcripts in eukaryotic cells. In this study, we investigate >1,100 unannotated yeast predicted to lack protein-coding capacity. We show that majority these RNAs are enriched on polyribosomes akin mRNAs. Ribosome profiling demonstrates many bind translocating ribosomes within open reading frames 10–96 codons size. validate peptides encoded subset and provide evidence for conservation among species. Consistent with their translation, targeted degradation by the translation-dependent nonsense-mediated RNA decay (NMD) pathway. identify lncRNAs also sensitive NMD, indicating translation noncoding occurs mammals. These data demonstrate considered coding potential bona fide protein expand proteome possibly other eukaryotes.
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