Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus
作者： Watt W. Webb
DOI: 10.1364/AO.40.003969
关键词: Molecule 、 Light scattering 、 Raman scattering 、 Optics 、 Fluorescence cross-correlation spectroscopy 、 Fluorescence spectroscopy 、 Fluorescence 、 Fluorescence correlation spectroscopy 、 Materials science 、 Infrared 、 Atomic and Molecular Physics, and Optics
摘要: Fluorescence correlation spectroscopy examines the chemical and the photophysical dynamics of dilute molecular solutions by measurement of the dynamic optical fluctuations fluorescence a few molecules, even averaging less than one molecule at time, in open focal volumes that are usually femtoliter (<10-18 m3). It applies same principles statistical thermodynamics as does quasi-elastic light scattering. Molecular interactions, conformational changes, chemical reactions, photophysical not ordinarily detectable light scattering can be analyzed by fluorescence cases which molecular fluorescence changes range 10-7–102 s.
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