Double-stranded Internucleosomal Cleavage of Apoptotic DNA Is Dependent on the Degree of Differentiation in Muscle Cells
作者： Gian Maria Fimia , Vanesa Gottifredi , Claudio Passananti , Rossella Maione
关键词: Biology 、 MyoD 、 Apoptotic DNA fragmentation 、 DNA fragmentation 、 Molecular biology 、 Endonuclease 、 Apoptosis 、 Cleavage (embryo) 、 Base pair 、 DNA 、 Cell biology 、 Biochemistry
摘要: Apoptotic cell death has been correlated to DNA fragmentation into discrete segments corresponding the length of nucleosomal protected fragments 180–200 base pairs or multiples it. This degradation ascribed endonuclease activity that cleaves internucleosomally, thus giving rise a ladder distribution upon electrophoretic migration. strict correlation was, however, shown have notable exceptions, since in some cases only single strand cleavage internucleosomal regions observed (Tomei, D. L., Shapiro, P. J., and Cope, O. F. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 853–857). In present work we show mouse muscle cells, able differentiate vitro, if subjected apoptosis no form unless differentiation is previously induced. Furthermore, C3H/10T1/2 fibroblast known undergo without formation, converted myogenic program by MyoD expression, display induction differentiation.
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