An electrospun fiber-covered stent with programmable dual drug release for endothelialization acceleration and lumen stenosis prevention.

摘要: Abstract Aneurysmal subarachnoid hemorrhage (SAH) causes high rates of mortality and morbidity. A covered stent is an effective endovascular treatment for complicated aneurysms intractable to coiling surgical clipping. However, in-stent restenosis delayed endothelialization are the main challenges contributing its safety. In this study, we designed a biofunctional with dual drug-loaded electrospun fibers achieve programmed vascular endothelial growth factor (VEGF) paclitaxel (PTX) release early promotion long-term inhibition stenosis caused by smooth muscle hyperplasia. By encapsulating PTX-loaded mesoporous silica nanoparticles (MSNs) within polylactic acid (PLA) fibers, period PTX was effectively extended. Furthermore, VEGF conjugated onto surface membrane reacting polydopamine (PDA) quick release. The in vitro drug profile revealed sustained PTX, which persisted 63 days without burst release, while up 87.05% rapidly released 3 days. After 6 days incubation, cell experiments demonstrated that scaffold prompted proliferation (488% vs. 386% control group, P = 0.001) inhibited cells (SMCs) using 21-day extracts (155% 303% P = 0.039). Animal studies showed compared bare stents, stents improved immediate- mid-term complete aneurysm occlusion (P  Statement Significance one most common stroke resulted nearly 40% 33% morbidity due sudden rupture intracranial aneurysm. Endovascular coil embolism popular but technique run risk bleeding, mass effect, low rate higher recanalization operation conducted sac. bio-functional knitted dual-drug loaded on realize programed endothelium This study provides new method improve biosafety insertion aneurysms.