111Indium Loss from Platelets by in Vitro and Ex Vivo Manipulation

作者: R. J. Hawker , C. E. Hall , M. Goldman , C. N. McCollum

DOI: 10.1007/978-94-009-6027-5_4

关键词: PlateletIn vitroIn vivoThrombogenicityLabellingIndiumChemistryEx vivoCellBiophysics

摘要: Since the introduction of lipid soluble chelates 111In as cell labelling agents (1) there have been a large number reports on in vivo and vitro uses Indium labeled platelets derived from man laboratory animals. Such labelled cells used for determination deep vein thrombosis (2) renal transplant rejection associated problems (3) detection thrombogenicity prosthetic arterial graft materials (4). Animal models to evaluate medical plastics (5) drug regimes (6) induced normalisation platelet survival (7). Laboratory assays developed utilize release human other quantification humoral or cellular antibodies (8,9). The advantages over 51Cr these tests not only relates 111In’s superior gamma emissions but excellent efficiency very low spontaneous release. is contained within storage granules (10) therefore granular initiated upon irreversible aggregation does cause loss Indium. This case with some species where stored released activation (11). There several quoting high levels which are found non-cell bound samples taken researchers suggest that may be re-utilized by blood-cells. Several authors thus advocated data should based radioactivity separated rather than whole blood-samples. involves great deal ex manipulations introduce their own errors create artifacts.

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