Magnetic resonance contrast and biological effects of intracellular superparamagnetic iron oxides on human mesenchymal stem cells with long-term culture and hypoxic exposure

摘要: Abstract Background aims Human mesenchymal stem cells (hMSCs) have gained interest for treatment of stroke injury. Using in vitro culture, the purpose this study was to investigate long-term detectability hMSCs by magnetic resonance imaging (MRI) after transfection with a superparamagnetic iron oxide (SPIO) and evaluate effects SPIO on cellular activity, particularly under an ischemic environment. Methods were exposed low doses SPIOs. After short incubation period, cultured additional 1, 7 14 d proliferation, colony formation multilinear potential. Labeled imaged evaluated agarose quantify R 2 ∗ contrast at each time point. Cells placed low-oxygen, low-serum environment tested cytotoxicity. In addition, labeled transplanted into model ex vivo MRI histology. Results Cellular events such as proliferation differentiation not affected any exposures when d. The exposure are sufficient MRI. However, higher dosage results calcification cytotoxicity conditions. Transplantation initial 22.4 μg Fe showed excellent retention stroke-induced rats. Conclusions Although labeling is stable detection has limited multilineage potential hMSCs, high-dose may affect hMSC survival serum oxygen withdrawal.