A Highly Selective, Biofunctional Surface for Molecule/Cell Sorting

摘要: We report in this paper an approach to the effective capture of IgM antibodies from antisera and solutions based on formation a carpet molecules exposing thiols off surface. Surfaces different nature, such as OH-exposing (glass, SiO(2), metal oxides, etc.) noble ones (Au, Ag, etc.), have been first functionalized liquid phase by suitable chemistry [3-(mercaptopropyl)trimethoxysilane or 1,4-benzenedimethanethiol]. The resulting exposed SH moieties further used for binding anti-A, -B, -D goat sera via thiol exchange reaction involving J chain other disulfide bonds present molecular structure. Antibodies preserve their functional activity at surface appear be able bind specifically erythrocytes proper group fast reliable way. These results can generalized use any kind antibody valuable biofunctionalization fields biosensors immunoassays.