pH- and salt-dependent molecular combing of DNA: experiments and phenomenological model.
作者： Annegret Benke , Michael Mertig , Wolfgang Pompe
DOI: 10.1088/0957-4484/22/3/035304
关键词: Substrate (chemistry) 、 DNA 、 Chemical engineering 、 Adhesion 、 Chemistry 、 Sticky and blunt ends 、 Hydrophobic effect 、 Molecule 、 Nanotechnology 、 A-DNA 、 Adsorption
摘要: λ-DNA as well plasmids can be successfully deposited by molecular combing on hydrophobic surfaces, for pH values ranging from 4 to 10. On polydimethylsiloxane (PDMS) substrates, the DNA molecules are overstretched about 60–100%. There is a significant influence of sodium ions (NaCl) surface density DNA, with maximum near 100 mM NaCl solution (28 ng µl − 1) at 8. The process described micromechanical model including: (i) the adsorption free moving coiled substrate; (ii) the stretching preceding meniscus; (iii) the relaxation final length. sticky ends cause an adhesion force in range 400 pN which allows stable overstretching meniscus. exposing hidden bonds leads deposition substrate. pH-dependent observed explained their screening negatively charged backbone and ends, respectively. length derived balance stored elastic energy adhesion.
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sci-hub.se 参考文章(26)
Frank Baldino, Marie-Francoise Chesselet, Michael E. Lewis, High-resolution in situ hybridization histochemistry. Methods in Enzymology. ,vol. 168, pp. 761- 777 ,(1989) , 10.1016/0076-6879(89)68057-3
G. Michael Blackburn, M. J. Gait, Nucleic Acids in Chemistry and Biology ,(1989)
Hermann E. Gaub, Matthias Rief, Hauke Clausen-Schaumann, Sequence-dependent mechanics of single DNA molecules Nature Structural & Molecular Biology. ,vol. 6, pp. 346- 349 ,(1999) , 10.1038/7582
A Bensimon, A Simon, A Chiffaudel, V Croquette, F Heslot, D Bensimon, Alignment and sensitive detection of DNA by a moving interface Science. ,vol. 265, pp. 2096- 2098 ,(1994) , 10.1126/SCIENCE.7522347
D. Bensimon, A. J. Simon, V. Croquette, A. Bensimon, Stretching DNA with a receding meniscus: Experiments and models. Physical Review Letters. ,vol. 74, pp. 4754- 4757 ,(1995) , 10.1103/PHYSREVLETT.74.4754
Minjung Shin, Chilwoo Kwon, Seong Kyu Kim, Hyung Jin Kim, Yonghan Roh, Byungyou Hong, Jong Bae Park, Haeseong Lee, Formation of λ-DNA's in Parallel- and Crossed-Line Arrays by Molecular Combing and Scanning-Probe Lithography Nano Letters. ,vol. 6, pp. 1334- 1338 ,(2006) , 10.1021/NL060160U
Hauke Clausen-Schaumann, Matthias Rief, Carolin Tolksdorf, Hermann E. Gaub, Mechanical Stability of Single DNA Molecules Biophysical Journal. ,vol. 78, pp. 1997- 2007 ,(2000) , 10.1016/S0006-3495(00)76747-6
Roland G. Winkler, Andrey G. Cherstvy, Adsorption of weakly charged polyelectrolytes onto oppositely charged spherical colloids. Journal of Physical Chemistry B. ,vol. 111, pp. 8486- 8493 ,(2007) , 10.1021/JP068489R
Sebastian Doniach, Biological Physics: Energy, Information, Life ,(2003)
Zhaoxiang Deng, Chengde Mao, DNA-Templated Fabrication of 1D Parallel and 2D Crossed Metallic Nanowire Arrays Nano Letters. ,vol. 3, pp. 1545- 1548 ,(2003) , 10.1021/NL034720Q