Improved production of gutted adenovirus in cells expressing adenovirus preterminal protein and DNA polymerase.

摘要: Production of gutted, or helper-dependent, adenovirus vectors by current methods is inefficient. Typically, a plasmid form the gutted genome transfected with helper viral DNA into 293 cells; resulting lysate serially passaged to increase titer virions. Inefficient production virus particles after cotransfection likely due suboptimal association replication factors abnormal origins found in these substrates. To test this hypothesis, we explored whether would be facilitated transfection cells expressing various factors. We observed that C7 cells, coexpressing adenoviral polymerase and preterminal protein, converted replicating approximately 50 times more efficiently than did cells. This property can used greatly efficiency DNA. These should also useful for generation recombinant from any plasmid-based precursor.