Development and characterization of single step self-assembled lipid polymer hybrid nanoparticles for effective delivery of methotrexate
作者： Neeraj K Garg , Bhupinder Singh , Gajanand Sharma , Varun Kushwah , Rajeev K Tyagi
DOI: 10.1039/C5RA12459J
关键词: Chemistry 、 Polymer 、 In vitro 、 Chromatography 、 Nanoparticle 、 Entrapment 、 Particle size 、 Pulmonary surfactant 、 Drug 、 Fourier transform infrared spectroscopy
摘要: The present study was designed to develop methotrexate (MTX) loaded lipid polymer hybrid nanoparticles (LPHNPs) for spatial and controlled delivery of this drug. LPHNPs were formulated by single step self-assembled nano-precipitation method. effect variables such as surfactants, varying surfactant concentration, phospholipids lipid–polymer ratio on particle size drug entrapment efficiencies systematically assessed optimize LPHNPs. found in nanometric range (150–300) with spherical shape. efficiency developed formulations calculated between 80 90%. into further validated FTIR XRD analysis. In vitro release showed slow sustained i.e. more than 80% at the end 7th day. followed Korsmeyer–Peppas model showing Fickian diffusion. cytotoxicity optimized MCF-7 cells MTT, immunofluorescence assay (IFA) well confocal laser scanning microscopy (CLSM). result obtained cell line studies suggested that LPHNP is a prominent vehicle MTX breast cancers.
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