Chemistry, Mass Spectrometry and Peptide-Mass Databases: Evolution of Methods for the Rapid Identification and Mapping of Cellular Proteins

摘要: Large-format 2D gel electrophoresis systems have been developed that are capable of resolving several thousand cellular proteins in a matter days [1,2]. For number years, combination Edman microsequence analysis and identification by staining with specific antibodies has used to systematically categorize establish databases [3, 4, 5]. There are, however, significant problems associated these approaches. Most resolved only present the low- upper-femtomole range, significantly below level at which automated sequencers can reliably operate [6, 7]. The relatively slow speed process (one or two samples per machine day) also means sheer is too great permit large-scale characterization within any useful period time. use monoclonal antibodies, whilst both rapid extremely sensitive, requires ready availability large pool antibody probes.