Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

摘要: There is an incentive to functionalise hydroxyapatite (HA) for orthopaedic implant use with bioactive agents encourage superior integration of the implants into host bone. One such agent (3S) 1-fluoro-3-hydroxy-4-(oleoyloxy) butyl-1-phosphonate (FHBP), a phosphatase-resistant lysophosphatidic acid (LPA) analogue. We investigated effect FHBP-HA coating on maturation human (MG63) osteoblast-like cells. Optimal conditions were identified and cell modified unmodified, control HA surfaces was assessed. Stress tests performed evaluate survivorship after exposure mechanical thermal insults that are routinely encountered in clinical environment. MG63 found be three times greater FHBP-modified compared controls (p < 0.0001). no significant loss bioactivity autoclaving (P = 0.9813) although functionality declined by 67% cleaning reuse The disks significantly than following storage up six months (p < 0.001). Herein we demonstrate can functionalised FHBP facile, scalable manner this novel surface has capacity enhance osteoblast maturation. Improving biological performance bone regenerative setting could realised through simple conjugation LPA species future.