Effect of recombinant erythropoietin on ischemia-reperfusion-induced apoptosis in rat liver.

摘要: Ischemia–reperfusion (I/R) cannot be avoided in liver transplantation procedures, and apoptosis is a central mechanism of cell death after reperfusion. Protective effect recombinant erythropoietin (rhEPO) on has not been clearly investigated. This work investigated intraportal (IP) rhEPO-protective rat model hepatic I/R-induced its appropriated time dose administration. Eight groups were included (n = 10/group): sham-operated, I/R (45 min ischemia 2 h reperfusion), preconditioned rhEPO (24 or 30 before ischemia), postconditioned (before reperfusion) using two different doses (1,000 5,000 IU/kg). When compared with the sham-operated group, group showed significant increase serum levels aspartate alanine aminotransferases (AST, ALT), caspase-9 activity(894.99 ± 176.90 relative fluorescence units (RFU)/mg/min versus 458.48 82.96 RFU/mg/min), Fas ligand (FasL) expression, histopathological damages, decrease antiapoptotic Bcl-xL/apoptotic Bax ratio(0.38 0.21 3.35 0.77) rhEPO-improved ALT AST but failed to reduce FasL expression all group. Thirty minutes 24 preconditioning IU/kg) increased Bcl-xL/Bax ratio reduced activity, same was observed when higher given ischemia. Preconditioning more effective than postconditioning improving no dose-dependent observed. In conclusion, single IP injection an advantage over post-hepatic additional time- effects which may provide potentially useful guide procedures.