The Dissociation by Colchicine of Phagocytosis from Increased Oxygen Consumption in Human Leukocytes*

摘要: The effect of colchicine on the uptake oxygen by human leukocytes during phagocytosis live streptococci or killed staphylococci was compared with per se, measured in a sensitive bacterial system. increase consumption that normally accompanies consistently diminished incubated concentrations as low 2.5 x 10(-6) mole L (1 mug ml), and this inhibition dosage dependent. Yet there no evidence decreased high 10(-4) (100 ml). Furthermore, measurements at 20, 40, 60 minutes, rate comparable without colchicine.A clue to dissociation between found rapidly dried preparations leukocytes. Ingested bacteria were present both control colchicine-treated granulocytes. In addition, cells showed normal loss granules (lysosomal particles) prominent cytoplasmic vacuoles (digestive vacuoles). Colchicine-treated cells, however, less such degranulation vacuolization. Measurements granule-associated acid phosphatase activity after support morphologic observations muted metabolic morpholgic response may be important for anti-inflammatory acute gouty arthritis. Colchicine also find wider use defining structure-function dependencies metabolically stimulated cells.