CENP-A Phosphorylation by Aurora-A in Prophase Is Required for Enrichment of Aurora-B at Inner Centromeres and for Kinetochore Function
作者： Naoko Kunitoku , Takashi Sasayama , Tomotoshi Marumoto , Dongwei Zhang , Shinobu Honda
DOI: 10.1016/S1534-5807(03)00364-2
关键词: Aster (cell biology) 、 Mitosis 、 Aurora B kinase 、 Cell biology 、 Biology 、 Prometaphase 、 Kinetochore 、 Prophase 、 Microtubule 、 Centromere
摘要: The Aurora (Ipl1)-related kinases are universal regulators of mitosis. We now show that Aurora-A, in addition to Aurora-B, regulates kinetochore function human cells. A two-hybrid screen identified the component CENP-A as a protein interacts with Aurora-A. Aurora-A phosphorylated vitro on Ser-7, residue also known be targeted by Aurora-B. Depletion or Aurora-B RNA interference revealed is initially prophase manner dependent and this reaction appears required for subsequent Aurora-B-dependent phosphorylation well restriction inner centromere prometaphase. Prevention led chromosome misalignment during mitosis result defect attachment microtubules. Our observations suggest Ser-7 essential function.
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