Isolation, identification and cyfluthrin-degrading potential of a novel Lysinibacillus sphaericus strain, FLQ-11-1

摘要: Abstract Strain FLQ-11-1, isolated from sewage sludge, was able to degrade cyfluthrin and identified as Lysinibacillus sphaericus based on its morphology, 16S rRNA sequence fatty acid methyl ester (FAME) analyses. This strain could use carbon or nitrogen source. Response surface methodology (RSM) analysis showed that the optimum conditions for degradation were at pH 7.0 35 °C, using an inoculum amount with OD 600nm value of 1.6. Under these conditions, approximately 80.4% (50 mg l −1 ) degraded within five days (d) incubation. Four metabolic compounds detected during methyl-3-(2,2-dichlorovinyl)-2,2-dimethyl-(1-cyclopropane)-carboxylate, 4-fluoro-3-phenoxy-benzoic ester, methyl-3-phenoxybenzoate, 3-phenoxy-benzaldehyde by gas chromatography–mass spectrometry (GC–MS) tandem mass spectrum (MS/MS) no after seven A possible pathway proposed, our data be efficiently indicating this potentially used eliminate contamination pyrethroid herbicides.