CD43 interferes with T-lymphocyte adhesion

摘要: CD43 is a cell-surface sialoglycoprotein of uncertain physiologic function expressed to various degrees by most leukocytes. We tested whether or not participates in intercellular adhesion comparing the binding human T lymphocytes transfected HeLa cells stably expressing and sham-transfected (CD43-negative). Significantly fewer adhered CD43-positive than CD43-negative cells. Diminished T-cell adherence was seen for all tested, irrespective their source derivation. Antibody-blocking experiments revealed that interference with largely represented leukocyte function-associated antigen 1 cell molecule 1. The anti-adhesion effect overcome treating phorbol 12-myristate 13-acetate, chemical increases avidity However, neuraminidase treatment transfectants diminished antiadhesion effect. These data indicate expression opposing can interfere cell-cell adhesion. also suggest might regulate interfering 1, major activation-induced pathway among lymphocytes.